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Calf-intestinal alkaline phosphatase : ウィキペディア英語版 | Calf-intestinal alkaline phosphatase Calf-intestinal alkaline phosphatase (CIAP/CIP) is a type of alkaline phosphatase that catalyzes the removal of phosphate groups from the 5' end of DNA strands.〔〔 This enzyme is frequently used in DNA sub-cloning, as DNA fragments that lack the 5' phosphate groups cannot ligate. 〔 This prevents recircularization of linearized vector DNA and improves the yield of vector containing the appropriate insert. ==References==
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「Calf-intestinal alkaline phosphatase」の詳細全文を読む
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